Image: Blots of mouse brain homogenates stained with Fox1. Antibody binds closely spaced 48kDa and 46kDa bands. http://www.neuromics.com/ittrium/visit/A1x66x1y1x85b1x1x9cy1x6217x1x96y1x71bbx1y1x71b8x1x82y1x8f74x1x7f
Blots of crude cow cerebellum homogenate blotted with Aldolase-C. The monoclonal antibody binds strongly and cleanly to a band at about 40 kDa. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.
Overview of actin gamma IP procedure using SureBeads Magnetic Beads followed by western blot detection of the IP sample with an anti-actin gamma primary antibody and a standard secondary antibody or TidyBlot. Using TidyBlot enables the detection of actin gamma without interference from the IgG heavy chain; the molecular weights of actin-gamma (46 kDa) and the IgG heavy chain (50 kDa) are very close, which often results in masking of the actin gamma band by the IgG heavy chain.