In Situ Hybridization (ISH) In Situ Hybridization (ISH) refers to localizing a specific DNA or RNA sequence in whole embryos or tissues/tissue sections/cells using a labeled probe. The probe is either a labeled complementary DNA (oligoprobe) or a complementary RNA (riboprobe).... http://www.creative-diagnostics.com/In-Situ-Hybridization-ISH.htm
Transcription proceeds in the following general steps: One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA. RNA polymerase creates a transcription bubble, which separates the two strands of the DNA helix. This is done by breaking the hydrogen bonds between complementary DNA nucleotides. RNA polymerase adds matching RNA nucleotides to the complementary nucleotides of one DNA strand.
Controlling the self-assembly of nanoparticles into superlattices is an important approach to build functional materials. The Brookhaven team used nanosized building blocks—cubes or octahedrons—decorated with DNA tethers to coordinate the assembly of spherical nanoparticles coated with complementary DNA strands.
The picture shows a microscope image of micrometer-sized flowers self-assembled from silica particles equipped with sticky “DNA-glue". The core of the flowers are 1 micrometer-sized silica particles functionalized with a DNA strand with a green dye. The petals of the flowers consist of 2 micrometer-sized particles, containing the complementary DNA strand with a red dye. Using these particles in different concentrations, beautiful flower-like structures form due to the DNA strands.
Central Dogma of Molecular Biology -DNA makes RNA -RNA makes Proteins -Proteins help DNA (and sometimes RNA) to replicate itself -And some Proteins, like those from retrovirus HIV can turn RNA back into DNA (called complementary DNA or cDNA for short)
In this craft-based activity, learners make DNA sequence bracelets that carry the code of an organism such as a human, trout, chimpanzee or butterfly. This activity reinforces the principle of complementary base pairs as learners are given one strand of the sequence and they have to match up the other strand correctly.